Return to MicroscopeMaster home. Methanobacteria is a class of the phylum Euryarchaeota within the domain Archaea. Read more here. The Islets of Langerhans is an endocrine tissue located within the pancreas. It consists of a variety of cells capable of producing different hormones. Hydrogen-oxidizing Bacteria are species that can use gaseous hydrogen as the electron donor to oxidize hydrogen.
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Scientific understanding changes over time. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. The MicroscopeMaster website is for educational purposes only. Amazon and the Amazon logo are trademarks of Amazon. Hektoen Agar. Hektoen - uninoculated. Purpose: Detects lactose fermentation, H 2 S production, inhibits non-enterics.
Interpretation: Lactose fermenters yellow or salmon, non-fermenters colorless; H 2 S production produces black precipitate. Hektoen - Escherichia coli Note: Orange color indicates acid production as a result of lactose fermentation. Mannitol Salt Agar. Mannitol Salt Agar - uninoculated. Purpose: Selects for Staphylococci, which grow at high salt concentrations; differentiates Staphylococcus aureus from other Staphylococci. Interpretation: Staphylococcus aureus is yellow ferments mannitol , other staphylococci are white.
Mannitol Salt Agar - Staphylococcus epidermidis Note: growth, but no fermentation of mannitol, medium color unchanged. Mannitol Salt Agar - Staphylococcus aureus Note: yellow color due to acid produced by fermentation of mannitol. Triple Sugar Iron Agar - uninoculated. Purpose: Detects glucose, lactose, sucrose fermentation; gas and H 2 S production. Triple Sugar Iron Agar - Escherichia coli. Triple Sugar Iron Agar - Salmonella.
Triple Sugar Iron Agar - Shigella. Triple Sugar Iron Agar - Pseudomonas aeruginosa. Hemolytic Reactions Observed on Blood Agar Observation of the hemolytic reactions on sheep blood agar is a very useful tool in the preliminary identification of bacteria, particularly streptococci.
Sheep Blood Agar - uninoculated. Alpha hemolysis. Sheep Blood Agar - Streptococcus pneumoniae , alpha hemolytic. Sheep Blood Agar - Streptococcus pyogenes , beta hemolytic. MacConkey agar plates MAC are a differential type medium made with bile salts and crystal violet. These additives cause the agar to only allow growth of Gram-negative bacteria, while inhibiting the growth of Gram-positive organisms.
This agar can also contain lactose to distinguish between lactose-fermenting and non-lactose fermenting bacteria by forming either red lactose-fermenting or clear colonies. Eosin Methylene Blue EMB agar plates do the same thing but use two dyes, eosin and methylene blue, to differentiate between the bacteria. There are many other types of agar plates that can select for or differentiate between specific species of bacteria or other microorganism.
Mannitol salt agar MSA selects for organisms that ferment mannitol by turning the plate yellow through a pH change. MSA also differentiates between the pathogenic and non pathogenic type of Staphylococci. Sabouraud dextrose agar SDA allows growth of fungi, yeasts and mold and has a low pH, preventing growth of bacteria. Sarah Quinlan has experience writing for various websites on science, biology, veterinary science, health and medicine.
For over seven years she has worked as a scientist in various biological fields where she has written and contributed to multiple manuscripts that have been published in scientific journals. The Chemical Composition of Nutrient Agar. Five Steps to Prepare Agar Slants.
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